| Species name: Canada thistle (Cirsium arvense (L.) Scop.)|
Canada thistle is a dioecious perennial that spreads both by seed and vegetatively by creeping roots (http://en.wikipedia.org/wiki/Cirsium_arvense). Recent classifications place it in subfamily Carduoideae, tribe Cardueae, and subtribe Centaureinae. The species is native to temperate regions of Eurasia and was introduced to North America in the 1600s. It is one of the most tenacious and damaging agricultural weeds in North America and appears on the noxious weed list of 43 states and six provinces (http://invader.dbs.umt.edu/Noxious_Weeds/noxlist.asp). Through competition for water, nutrients and minerals, Canada thistle can generate greater crop losses per unit area than any other broadleaf weed. The species is considered to be diploid, with a gametic chromosome number of 17 (http://www.tropicos.org/Project/IPCN) and a genome size of circa 1,390 Mbp (http://data.kew.org/cvalues/).
- Nucleotide sequences (57 total) on GenBank as of 12/2012: available here
- ESTs (173 Sanger reads) on GenBank as of 12/2012: available here
- EST library for invasive genotype based on 454 sequencing (1,411 Mbp): available online.
- EST library for invasive genotype based on Illumina sequencing (2,988 Mbp): available online.
- EST library for native genotype based on Illumina sequencing (2,995 Mbp): available online.
- 12-plex NimbleGen microarray (63,690 unigenes; 136,582 features)
| Images of Cirsium arvense|
| Figure 1: Photo by Thomas Nugent; licensed for re-use under the Creative Commons Attribution-Share Alike License.||
| Figure 2: Photo by André Karwath; licensed for re-use under the Creative Commons Attribution-Share Alike License.||
| CGP Activities|
We generated the 454 and Illumina EST libraries and NimbleGen microarray described above in collaboration with an NSERC-funded project on Compositae weeds. We are collaborating with the latter project on an analysis of expression differences between native and invasive populations of the species.
Lai Z, NC Kane, A Kozik, KA Hodgins, KM Dlugosch, MS. Barker, M Matvienko, Q Yu, KG Turner, SA Pearl, GDM Bell, Y Zou, C Grassa, A Guggisberg, KL Adams, JV Anderson, DP Horvath, RV Kesseli, JM Burke, RW Michelmore, and LH Rieseberg. 2012. Genomics of Compositae weeds: EST libraries, microarrays, and evidence of introgression. American Journal of Botany, In review.